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Improvement of the biocatalytic properties of one phenylacetone monooxygenase mutant in hydrophilic organic solvents

Paper ID Volume ID Publish Year Pages File Format Full-Text
17344 42661 2012 7 PDF Available
Title
Improvement of the biocatalytic properties of one phenylacetone monooxygenase mutant in hydrophilic organic solvents
Abstract

The presence of different hydrophilic organic solvents or a water soluble polymer such as PEG 4000 led to an enhancement in the enzymatic activity of the M446G mutant of phenylacetone monooxygenase when it is employed in enantioselective sulfoxidations and Baeyer–Villiger reactions. By solvent engineering new substrates were found to be effectively converted by this Baeyer–Villiger monooxygenase. The use of 5% methanol together with the weak anion exchange resin Lewatit MP62 also allows the dynamic kinetic resolution of a set of racemic benzylketones. By this approach (S)-benzylesters could be obtained with high yields and optical purities.

► The effect of organic solvents on M446G mutant of phenylacetone monooxygenase was examined. ► Presence of hydrophilic solvents led to an increase in the enzymatic activity. ► The use of 5% methanol combined with anion exchange resins allowed DKR processes.

Keywords
Baeyer–Villiger monooxygenases; Sulfoxidations; Solvent engineering; Baeyer–Villiger oxidation; Dynamic kinetic resolution
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Improvement of the biocatalytic properties of one phenylacetone monooxygenase mutant in hydrophilic organic solvents
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 50, Issue 1, 5 January 2012, Pages 43–49
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us