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Production of l-xylose from l-xylulose using Escherichia colil-fucose isomerase

Paper ID Volume ID Publish Year Pages File Format Full-Text
17348 42661 2012 6 PDF Available
Title
Production of l-xylose from l-xylulose using Escherichia colil-fucose isomerase
Abstract

l-Xylulose was used as a raw material for the production of l-xylose with a recombinantly produced Escherichia colil-fucose isomerase as the catalyst. The enzyme had a very alkaline pH optimum (over 10.5) and displayed Michaelis–Menten kinetics for l-xylulose with a Km of 41 mM and a Vmax of 0.23 μmol/(mg min). The half-lives determined for the enzyme at 35 °C and at 45 °C were 6 h 50 min and 1 h 31 min, respectively. The reaction equilibrium between l-xylulose and l-xylose was 15:85 at 35 °C and thus favored the formation of l-xylose. Contrary to the l-rhamnose isomerase catalyzed reaction described previously [14]l-lyxose was not detected in the reaction mixture with l-fucose isomerase. Although xylitol acted as an inhibitor of the reaction, even at a high ratio of xylitol to l-xylulose the inhibition did not reach 50%.

► l-Fucose isomerase catalyzed isomerization of l-xylulose to l-xylose does not produce l-lyxose. ► New characteristics on the catalytic mechanisms related to l-fucose isomerase are presented. ► A potential pathway for the production of the pharmaceutically important intermediate l-xylose is presented.

Keywords
l-Xylose; l-Xylulose; l-Fucose isomerase; Isomerization; Rare sugars
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Production of l-xylose from l-xylulose using Escherichia colil-fucose isomerase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 50, Issue 1, 5 January 2012, Pages 71–76
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us