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Bioremediase a unique protein from a novel bacterium BKH1, ushering a new hope in concrete technology

Paper ID Volume ID Publish Year Pages File Format Full-Text
17357 42662 2010 7 PDF Available
Title
Bioremediase a unique protein from a novel bacterium BKH1, ushering a new hope in concrete technology
Abstract

A novel hot spring bacterial strain was isolated and characterized from Bakreshwar, West Bengal, India. Phylogenetic analysis of partial 16 S-rRNA gene sequence (697 nucleotides) suggested that the isolate was affiliated with the family ‘Firmicutes’ and 99.8% homologous with Thermoanaerobactor thermohydrosulfuricus. A unique thermostable and high pH tolerant protein (28 kDa) secreted by the bacterium was isolated whose N-terminal partial sequence (25 AA) suggested that the protein was similar to mammalian carbonic anhydrase-II. This bacterium or its unique protein both increase the compressive (>25%) and tensile (>20%) strengths, hardness and elastic modulus of cementitious material when incorporated to the cement/cement–sand mixture. The protein (named as bioremediase) possesses silica leaching activity, which is increased by calcium ions. No esterase and/or proteolytic activities are present in the protein. The use of such isolated bacterium or its protein in concrete technology develops new construction material that may be used for self-healing concrete in future.

Keywords
Bioconcrete; Compressive strength; Hot spring bacteria; Mortar; Protein; Silica
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Bioremediase a unique protein from a novel bacterium BKH1, ushering a new hope in concrete technology
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 46, Issue 7, 7 June 2010, Pages 581–587
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us