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Immobilization of lysine oxidase on a gold–platinum nanoparticles modified Au electrode for detection of lysine

Paper ID Volume ID Publish Year Pages File Format Full-Text
17371 42663 2013 7 PDF Available
Title
Immobilization of lysine oxidase on a gold–platinum nanoparticles modified Au electrode for detection of lysine
Abstract

A commercial lysine oxidase (LyOx) from Trichoderma viride was immobilized covalently onto gold nanoparticles (AuNPs) and platinum nanoparticles (PtNPs) electrodeposited onto Au electrode using 3-aminopropyltriethoxy silane (3-APTES) and glutaraldehyde cross linking chemistry. A lysine biosensor was fabricated using LyOx/3-APTES/AuNPs-PtNPs/Au electrode as a working electrode, Ag/AgCl (3 M KCl) as standard electrode and Pt wire as auxiliary electrode connected through a potentiostat. The enzyme electrode was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The cumulative effect of AuNPs and PtNPs showed excellent electrocatalytic activity at low applied potential for detection of H2O2, a product of LyOx reaction. The sensor showed its optimum response within 4 s, when polarized at 0.2 V vs. Ag/AgCl in 0.1 M phosphate buffer, pH 7.5 at 30 °C. The linear range and detection limit of the sensor were 1.0–600 μM and 1.0 μM (S/N = 3), respectively. Biosensor measured lysine level in sera, milk and amino acid tablet, which correlated well with those by standard HPLC method. The enzyme electrode lost 50% of its initial activity after 200 uses over a period of 4 months.

► Developed a lysine biosensor based on 3-APTES/AuNPs-PtNPs/Au electrode. ► Biosensor showed maximum response within 4 s at 0.2 V, pH 7.5 and 30 °C. ► Biosensor had linear range 1.0–600 μM with a low detection limit of 1.0 μM. ► Half life of enzyme electrode was 4 months, after 200 uses.

Keywords
l-Lysine biosensor; Lysine oxidase; Gold nanoparticles; Platinum nanoparticles; 3-Aminopropyltriethoxy silane
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Immobilization of lysine oxidase on a gold–platinum nanoparticles modified Au electrode for detection of lysine
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 52, Issues 4–5, 10 April 2013, Pages 265–271
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us