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Process development of succinic acid production by Escherichia coli NZN111 using acetate as an aerobic carbon source

Paper ID Volume ID Publish Year Pages File Format Full-Text
17389 42665 2011 6 PDF Available
Title
Process development of succinic acid production by Escherichia coli NZN111 using acetate as an aerobic carbon source
Abstract

Escherichia coli strain NZN111 could convert glucose to succinic acid efficiently in anaerobic conditions after the induction of gluconeogenic carbon sources in aerobic conditions. Acetate shows a strong effect on both yield and productivity of succinic acid. In this study, the fed-batch process of succinic acid production by NZN111 using acetate in a chemically defined medium in the aerobic stage was investigated and developed. Increasing cell density could increase succinic acid with a productivity of 3.97 g/(L h) in the first 8 h of the anaerobic phase with an overall yield of 1.42 mol/mol glucose in a 5 L fermentor. However, there was strong repression from succinic acid in the later anaerobic stage. When succinic acid exceeded 30 g/L, the glucose consumption rate began to drop sharply along with the succinic acid production rate. Supplementation with glucose from 30 to 70 g/L in the anaerobic stage showed little effect on succinic acid production. Acetic acid and pyruvic acid accumulated had no effect on succinic acid formation because of their low concentration. With acetate as the sole carbon source for aerobic cultivation in the following scale-up, 60.09 g/L of succinic acid was produced with a yield of 1.37 mol/mol in a 50 L bioreactor.

► The fed-batch production of succinic acid by Escherichia coli NZN111 was investigated. ► Acetate was used in a chemically defined medium in the aerobic stage. ► Succinic acid over 30 g/L has strong repression on glucose consumption. ► 60.09 g/L of succinic acid was produced with a yield of 1.37 mol/mol in a 50 L bioreactor.

Keywords
Escherichia coli; Succinic acid; Acetate; Chemically defined medium; Repression; Scale-up
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Process development of succinic acid production by Escherichia coli NZN111 using acetate as an aerobic carbon source
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 49, Issue 5, 10 October 2011, Pages 459–464
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us