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Production of extremely pure diacylglycerol from soybean oil by lipase-catalyzed glycerolysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
17405 42666 2011 5 PDF Available
Title
Production of extremely pure diacylglycerol from soybean oil by lipase-catalyzed glycerolysis
Abstract

Research work was objectively targeted to synthesize highly pure diacylglycerol (DAG) with glycerolysis of soybean oil in a solvent medium of t-butanol. Three commercial immobilized lipases (Lipozyme RM IM, Lipozyme TL IM and Novozym 435) were screened, and Novozym 435 was the best out of three candidates. Batch reaction conditions of the enzymatic glycerolysis, the substrate mass ratio, the reaction temperature and the substrate concentration, were studied. The optimal reaction conditions were achieved as 6.23:1 mass ratio of soybean oil to glycerol, 40% (w/v) of substrate concentration in t-butanol and reaction temperature of 50 °C. A two-stage molecular distillation was employed for purification of DAG from reaction products. Scale-up was attempted based on the optimized reaction conditions, 98.7% (24 h) for the conversion rate of soybean oil, 48.5% of DAG in the glycerolysis products and 96.1% for the content of DAG in the final products were taken in account as the results.

Keywords
Glycerolysis; Diacylglycerols; Immobilized lipase; Soybean oil
First Page Preview
Production of extremely pure diacylglycerol from soybean oil by lipase-catalyzed glycerolysis
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 49, Issue 2, 10 July 2011, Pages 192–196
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering