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Improved chemical and physical stability of laccase after spherezyme immobilisation

Paper ID Volume ID Publish Year Pages File Format Full-Text
17418 42667 2009 4 PDF Available
Title
Improved chemical and physical stability of laccase after spherezyme immobilisation
Abstract

Laccase was successfully self-immobilised into particles using spherezyme methodology, with a retention of 53.9% of original enzyme activity. Post-treatment of the spheres with ethanolamine or a combination of polyethlyeneimine and ethanolamine reduced this to 49.4% and 23.3%, respectively. However, polyethyleneimine coated spherezymes displayed improved stability towards oxidative denaturation by mediators with improvements of 5.61-, 6.95- and 15.55-fold for 2-hydroxyphtalimide, N-hydroxybenzotriazole and 2,2,6,6-tetramethylpiperidinooxy free radical, respectively. The immobilised preparation displayed enhanced stability during temperature incubation at 60–70 °C with an improvement of 2.04- and 4.03-fold respectively for ethanolamine post-treated spherezymes and polyethyleneimine coated spherezymes. Furthermore, enhanced stability at acidic pH was observed, with the most pronounced enhancement of 2.49-fold obtained at pH 2, and 1.69 and 1.38 at pH 3 and 4, respectively.

Keywords
Laccase; Mediator; Immobilisation; Spherezymes; Stability
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Improved chemical and physical stability of laccase after spherezyme immobilisation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 45, Issues 6–7, December 2009, Pages 432–435
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us