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Amperometric biosensor based on enzymes immobilized in hybrid mesoporous membranes for the determination of acetylcholine

Paper ID Volume ID Publish Year Pages File Format Full-Text
17420 42667 2009 6 PDF Available
Title
Amperometric biosensor based on enzymes immobilized in hybrid mesoporous membranes for the determination of acetylcholine
Abstract

Acetylcholine sensor is successfully prepared by using immobilized enzymes, i.e., acetylcholinesterase and choline oxidase within separate hybrid mesoporous silica membranes with 12 nm pore diameter (F127M). The measurement was based on the detection of hydrogen peroxide produced by two sequential enzyme reactions. The determination range and the response time are 6.0–800 μM and within approximately 3 min, respectively. The sensor is very stable compared to free enzymes and 80% of the initial response was maintained even after storage for 80 days. These results show that two enzymes are successfully immobilized and well stabilized, and at the same time, two sequential enzyme reactions efficiently proceed within the separate hybrid mesoporous membranes. Further, we studied the possible detection of organophosphorus pesticides in terms of the inhibition of acetylcholinesterase activity, i.e., the decrease of current response, and demonstrated that the nanomolar concentrations of pesticide (DZN-oxon) can be detected with our sensor.

Keywords
Biosensor; Enzyme immobilization; Acetylcholinesterase; Choline oxidase; Sequential enzyme reaction; Hybrid mesoporous silica membrane; Inhibition; Pesticide detection
First Page Preview
Amperometric biosensor based on enzymes immobilized in hybrid mesoporous membranes for the determination of acetylcholine
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 45, Issues 6–7, December 2009, Pages 443–448
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering