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Robust production of gamma-amino butyric acid using recombinant Corynebacterium glutamicum expressing glutamate decarboxylase from Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
17439 42668 2012 6 PDF Available
Title
Robust production of gamma-amino butyric acid using recombinant Corynebacterium glutamicum expressing glutamate decarboxylase from Escherichia coli
Abstract

Gamma-amino butyric acid (GABA) is a component of pharmaceuticals, functional foods, and the biodegradable plastic polyamide 4. Here, we report a simple and robust system to produce GABA from glucose using the recombinant Corynebacterium glutamicum strain GAD, which expresses GadB, a glutamate decarboxylase encoded by the gadB gene of Escherichia coli W3110. As confirmed by HPLC analysis, GABA fermentation by C. glutamicum GAD cultured at 30 °C in GABA Production 1 (GP1) medium containing 50 g/L glucose without the addition of glutamate yielded 8.07 ± 1.53 g/L extracellular GABA after 96 h. Addition of 0.1 mM pyridoxal 5′-phosphate (PLP) was found to enhance the production of GABA, whereas Tween 40 was unnecessary for GABA fermentation. Using the optimized GABA Production 2 (GP2) medium, which contained 50 g/L glucose and 0.1 mM PLP, fermentation was performed in a flask at 30 °C with 10% (v/v) seed culture of C. glutamicum GAD. GABA was produced in the culture supernatant with a yield of 12.37 ± 0.88 g/L after 72 h with a space–time yield of 0.172 g/L/h, which is the highest yield obtained to date for GABA from fermentation with glucose as a main carbon source.

► GABA was produced directly from glucose by C. glutamicum expressing E. coli GadB. ► Without added glutamate, GABA productivity reached 12.3 g/L in culture supernatant. ► Addition of PLP to the medium was found to enhance the production of GABA. ► By using C. glutamicum GAD, GABA was produced in a maximum yield of 0.17 g/L/h.

Keywords
BHI, brain heart infusion; GABA, gamma-amino butyric acid; GAD, glutamate decarboxylase; LB, Luria–Bertani; MSG, monosodium glutamate; PA4, polyamide 4; PLP, pyridoxal 5′-phosphate; Tween 40, polyoxyethylene sorbitan monopalmitateCorynebacterium glutamicu
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Robust production of gamma-amino butyric acid using recombinant Corynebacterium glutamicum expressing glutamate decarboxylase from Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 51, Issue 3, 10 August 2012, Pages 171–176
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us