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Triphenylmethane dyes, an alternative for mediated electronic transfer systems in glucose oxidase biofuel cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
17460 42670 2011 11 PDF Available
Title
Triphenylmethane dyes, an alternative for mediated electronic transfer systems in glucose oxidase biofuel cells
Abstract

The bioelectrochemical behavior of three triphenylmethane (TPM) dyes commonly used as pH indicators, and their application in mediated electron transfer systems for glucose oxidase bioanodes in biofuel cells was investigated. Bromophenol Blue, Bromothymol Blue, Bromocresol Green were compared bioelectrochemically against two widely used mediators, benzoquinone and ferrocene carboxy aldehyde. Biochemical studies were performed in terms of enzymatic oxidation, enzyme affinity, catalytic efficiency and co-factor regeneration. The different features of the TPM dyes as mediators are determined by the characteristics in the oxidation/reduction processes studied electrochemically. The reversibility of the oxidation/reduction processes was also established through the dependence of the voltammetric peaks with the sweep rates. All three dyes showed good performances compared to the FA and BQ when evaluated in a half enzymatic fuel cell. Potentiodynamic and power response experiments showed maxima power densities of 32.8 μW cm−2 for ferrocene carboxy aldehyde followed by similar values obtained for TPM dyes around 30 μW cm−2 using glucose and mediator concentrations of 10 mmol L−1 and 1.0 mmol L−1, respectively. Since no mediator consumption was observed during the bioelectrochemical process, and also good redox re-cycled processes were achieved, the use of triphenylmethane dyes is considered to be promising compared to other mediated systems used with glucose oxidase bioanodes and/or biofuel cells.

Keywords
ABTS, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid); BCG, Bromocresol Green; BPB, Bromophenol Blue; BQ, p-benzoquinone; BTB, Bromothymol Blue; D, diffusion coefficient; DET, direct electronic transfer; E, potential; Eo′, formal potential; Epa, an
First Page Preview
Triphenylmethane dyes, an alternative for mediated electronic transfer systems in glucose oxidase biofuel cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 48, Issues 6–7, 6 May 2011, Pages 487–497
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering