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Evaluation of whole lysosomal enzymes directly immobilized on titanium (IV) oxide used in the development of antimicrobial agents

Paper ID Volume ID Publish Year Pages File Format Full-Text
17482 42672 2011 6 PDF Available
Title
Evaluation of whole lysosomal enzymes directly immobilized on titanium (IV) oxide used in the development of antimicrobial agents
Abstract

Lysosomal enzymes isolated from egg white were directly immobilized on titanium (IV) oxide (TiO2) particles using shaking methods (150 rpm, room temperature, 10 min), and the immobilization efficiency, activity, and stability of lysosomal enzymes immobilized on TiO2 were evaluated. Of the various mass ratios (w/w) of lysosomal enzymes to TiO2 tested, we found that 100% immobilization efficiency was observed at a ratio of 1:20 (enzymes:TiO2; w/w). Furthermore, the antimicrobial activities of the immobilized lysosomal enzymes were confirmed using viable cell counts against Escherichia coli. Our results showed that the antimicrobial activity of immobilized lysosomal enzymes is stable and can be maintained up to one month, but the antimicrobial activity of free enzymes without immobilization completely disappeared after five days in storage. In addition, enhanced immobilization efficiency was shown in TiO2 pretreated with a divalent, positively charged ion, Ca2+, and the antimicrobial activity for E. coli increased as a function of increasing ratio of immobilized enzymes. However, K+, a monovalent, positively charged ion, did not have any positive effect on immobilization or antimicrobial activity. Finally, we suggest that activity and stability of immobilized lysosomal enzymes can be maintained for a longer time than those properties of free lysosomal enzymes.

► Lysosomal enzymes isolated from egg immobilized on TiO2 using shaking method were evaluated immobilization efficiency with reaction time and various mass ratios. ► Antimicrobial activity of lysosomal enzymes immobilized on TiO2 was confirmed using viable cell counts against Escherichia coli and was stable and can be maintained up to one month. ► In addition, enhanced immobilization efficiency was shown in TiO2 pretreated with a divalent, positively charged ion, Ca2+, and the antimicrobial activity for E. coli increased as a function of increasing ratio of immobilized lysosomal enzymes.

Keywords
Lysosomal enzymes; TiO2; Direct immobilization; Antimicrobial activity; Divalent positively charged ion
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Evaluation of whole lysosomal enzymes directly immobilized on titanium (IV) oxide used in the development of antimicrobial agents
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 49, Issue 3, 10 August 2011, Pages 260–265
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us