fulltext.study @t Gmail

Electrochemical detection of Pseudomonas aeruginosa 16S rRNA using a biosensor based on immobilized stem–loop structured probe

Paper ID Volume ID Publish Year Pages File Format Full-Text
17483 42672 2011 6 PDF Available
Title
Electrochemical detection of Pseudomonas aeruginosa 16S rRNA using a biosensor based on immobilized stem–loop structured probe
Abstract

We have designed an electrochemical DNA biosensor based on stem–loop structured probes for enzymatic detection of Pseudomonas aeruginosa 16S ribosomal RNA (rRNA) in composting degradation. The probe modified with a thiol at its 5′ end and a biotin at its 3′ end was immobilized on a gold electrode through self-assembly. The stem–loop structured probes were “closed” when target was absent, then the hybridization of the target induced the conformational changes to “open”, along with the biotin at its 3′ end binding with streptavidin-horseradish peroxidase (HRP), and subsequent quantification of the target was detected via electrochemical detecting the enzymatic product in the presence of substrate. Under the optimum experiment conditions, the amperometric current response to HRP-catalyzed reaction was linearly related to the logarithm of the target nucleic acid concentration, ranging from 0.3 and 600 pg/μL, with the detection limit of 0.012 pg/μL. A correlation coefficient of 0.9960 was identified. The 16S rRNA extracted from P. aeruginosa was analyzed by this proposed sensor. The results were in agreement with the reference values deduced from UV spectrometric data. The biosensor was indicative of good precision, stability, sensitivity, and selectivity.

► An electrochemical DNA biosensor based on stem-loop structured probes has been designed. ► The biosensor with the detection limit of 0.012 pg/μL. ► 16S rRNA extracted from Pseudomonas aeruginosa was analyzed by this sensor. ► Results were in agreement with the reference values deduced from UV spectrometric data.

Keywords
Biosensor; Pseudomonas aeruginosa 16S rRNA; Stem–loop structured probe; Conformational changes; Electrochemical detection; Composting degradation
First Page Preview
Electrochemical detection of Pseudomonas aeruginosa 16S rRNA using a biosensor based on immobilized stem–loop structured probe
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 49, Issue 3, 10 August 2011, Pages 266–271
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us