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Evaluation of the xylan breakdown potential of eight mesophilic endoxylanases

Paper ID Volume ID Publish Year Pages File Format Full-Text
17489 42672 2011 7 PDF Available
Title
Evaluation of the xylan breakdown potential of eight mesophilic endoxylanases
Abstract

In biomass degradation using simultaneous saccharification and fermentation (SSF), there is a need for efficient biomass degrading enzymes that can work at lower temperatures suitable for yeast fermentation. As xylan is an important lignocellulosic biomass constituent, this study aimed at investigating the possible differences in xylan breakdown potential of endoxylanases using eight different endoxylanases at conditions relevant for SSF. Both solubilising and degrading capacities of the endoxylanases were investigated using water-insoluble and water-soluble oat spelt xylan as model substrates for biomass xylan. Results showed that selecting for combinations of endoxylanases that are efficient at solubilising xylan on the one hand and degrading it to large extent on the other hand, coupled to high specific activities, seems the best option for complete xylan breakdown in lignocellulosic biomass conversion using SSF.

Keywords
ARX,WS-X, endoxylanase activity towards WS-X based on the formation of reducing xylose; ARX,WI-X, endoxylanase activity towards WI-X based on the formation of reducing xylose; ASX,WI-X, endoxylanase solubilising activity on WI-X based on the formation of
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Evaluation of the xylan breakdown potential of eight mesophilic endoxylanases
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 49, Issue 3, 10 August 2011, Pages 305–311
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us