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Direct and efficient ethanol production from high-yielding rice using a Saccharomyces cerevisiae strain that express amylases

Paper ID Volume ID Publish Year Pages File Format Full-Text
17505 42673 2011 4 PDF Available
Title
Direct and efficient ethanol production from high-yielding rice using a Saccharomyces cerevisiae strain that express amylases
Abstract

Efficient ethanol producing yeast Saccharomyces cerevisiae cannot produce ethanol from raw starch directly. Thus the conventional ethanol production required expensive and complex process. In this study, we developed a direct and efficient ethanol production process from high-yielding rice harvested in Japan by using amylase expressing yeast without any pretreatment or addition of enzymes or nutrients. Ethanol productivity from high-yielding brown rice (1.1 g/L/h) was about 5-fold higher than that obtained from purified raw corn starch (0.2 g/L/h) when nutrients were added. Using an inoculum volume equivalent to 10% of the fermentation volume without any nutrient supplementation resulted in ethanol productivity and yield reaching 1.2 g/L/h and 101%, respectively, in a 24-h period. High-yielding rice was demonstrated to be a suitable feedstock for bioethanol production. In addition, our polyploid amylase-expressing yeast was sufficiently robust to produce ethanol efficiently from real biomass. This is first report of direct ethanol production on real biomass using an amylase-expressing yeast strain without any pretreatment or commercial enzyme addition.

Keywords
High-yielding rice; Amylase-expressing yeast; Ethanol
First Page Preview
Direct and efficient ethanol production from high-yielding rice using a Saccharomyces cerevisiae strain that express amylases
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 48, Issues 4–5, 7 April 2011, Pages 393–396
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering