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Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities

Paper ID Volume ID Publish Year Pages File Format Full-Text
17516 42674 2010 4 PDF Available
Title
Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities
Abstract

Long isomalto-oligosaccharides (IMOs) were generated via an engineered fusion enzyme of dextransucrase and dextranase (DSXR). To increase the expression level, response surface methodology (RSM) was utilized for optimization of protein expression conditions for enhancement of protein production by the effects of three-level-three-factors and their mutual interaction in Escherichia coli. Seventeen experiments were designed and conducted for investigation of cell density to start induction, induction temperature, and induction time. Optimal induction conditions included a cell density to start induction (A600) of 0.76 at 12.16 °C for 18 h for dextransucrase activity and a cell density to start induction (A600) of 0.75 at 10.5 °C for 20.9 h for dextranase activity. The produced dextransucrase or dextranase activity was obtained at 120.1 ± 7.2 or 871 ± 58 U, respectively, from 1 L cultures.

Keywords
Dextranase; Dextransucrase; Fusion enzyme; Isomalto-oligosaccharide; Response surface methodology
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Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 47, Issue 5, 6 October 2010, Pages 212–215
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us