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Property control of enzyme coatings on polymer nanofibers by varying the conjugation site concentration

Paper ID Volume ID Publish Year Pages File Format Full-Text
17517 42674 2010 6 PDF Available
Title
Property control of enzyme coatings on polymer nanofibers by varying the conjugation site concentration
Abstract

Trypsin was coated onto electrospun polystyrene–poly(styrene-co-maleic anhydride) (PS–PSMA) nanofibers with varied concentrations of maleic anhydride group, which enables an easy conjugation of enzymes onto PS–PSMA nanofibers. The concentration of maleic anhydride group, determined by the amount of PSMA in the mixture of PS and PSMA for electrospinning, correlated well with the enzyme loading and activity of covalently attached trypsin. Trypsin-coated nanofibers, prepared via covalent attachment of seed enzyme molecules and follow-up enzyme cross-linking, resulted in highly stable nanobiocatalytic systems, irrespective of added PSMA amounts. The variation of PSMA amounts enables the facile control of various properties of trypsin-coated nanofibers, such as enzyme loading, activity, structure, and mass transfer limitation. Especially, the mass transfer limitation of substrate through the enzyme coating matrix correlated well with the conjugation site concentration, and this feature would be useful in optimizing the enzyme coatings for the biocatalytic conversion of large-size substrates, such as protein digestion as an example. Facile property control of highly stable enzyme coatings would provide a powerful tool for more successful applications of enzyme coatings in various fields.

Keywords
Enzyme coatings; Covalent enzyme attachment; Trypsin; Electrospun polymer nanofibers; Conjugation site concentration; Mass transfer limitation
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Property control of enzyme coatings on polymer nanofibers by varying the conjugation site concentration
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 47, Issue 5, 6 October 2010, Pages 216–221
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us