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Production and optimization of recombinant human augmenter of liver regeneration by Pichia pastoris

Paper ID Volume ID Publish Year Pages File Format Full-Text
17518 42674 2010 6 PDF Available
Title
Production and optimization of recombinant human augmenter of liver regeneration by Pichia pastoris
Abstract

In this study, the fermentation of recombinant Pichia pastoris, which expresses heterogeneous human augmenter of liver regeneration (rhALR), has been optimized in a 5-L fermentor. The optimal methanol feeding method to optimize rhALR production was identified. Three methods of methanol feeding, methanol-stat, DO-stat and exponential feeding, were investigated to identify the method that would optimize rhALR production. It was determined that the methanol-stat method resulted in increased rhALR production, compared to the other two methods, which limit the amount of methanol available. Additionally, the results showed that rhALR expression levels could be enhanced by maintaining the pH at 6 and lowering the temperature from 30 to 25 °C. Supplementation with riboflavin during the induction phase also increased rhALR production. After these optimizations, rhALR was produced at a concentration of 424 mg/L, a three-fold improvement over original process.

Keywords
Pichia pastoris; Human augmenter of liver regeneration; Methanol feeding strategy; High cell density fermentation
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Production and optimization of recombinant human augmenter of liver regeneration by Pichia pastoris
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 47, Issue 5, 6 October 2010, Pages 222–227
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us