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Electroenzymatic synthesis of (S)-styrene oxide employing zinc oxide/carbon black composite electrode

Paper ID Volume ID Publish Year Pages File Format Full-Text
17524 42675 2010 9 PDF Available
Title
Electroenzymatic synthesis of (S)-styrene oxide employing zinc oxide/carbon black composite electrode
Abstract

(S)-styrene oxide, a useful chiral intermediate, has been synthesized using an electroenzymatic method with direct electrochemical FADH2 regeneration. Low electroenzymatic efficiency arising from the fast FADH2 reoxidation could be overcome by employing a zinc oxide/carbon black composite electrode. The attractive interaction between zinc oxide and styrene monooxygenase kept the local enzyme concentration high near the electrode surface, thereby increasing the accessibility of FADH2 from the electrode surface to the enzyme. By adjusting the reaction conditions such as oxygen solubility, a high electroenzymatic efficiency of 65% was obtained. As a result, the reaction rate was increased while the amount of the side-products from the cofactor reoxidation process was decreased. The metal oxide/carbon black composite electrode can be efficiently used for electroenzymatic syntheses using diffusible-flavin dependent monooxygenases.

Keywords
Electrochemical cofactor regeneration; Flavin cofactor; Styrene monooxygenase; (S)-styrene oxide; Electroenzymatic efficiency; Zinc oxide/carbon black
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Electroenzymatic synthesis of (S)-styrene oxide employing zinc oxide/carbon black composite electrode
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 47, Issue 7, 8 December 2010, Pages 313–321
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us