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Behavior of different monocomponent endoglucanases on the accessibility and reactivity of dissolving-grade pulps for viscose process

Paper ID Volume ID Publish Year Pages File Format Full-Text
17530 42675 2010 8 PDF Available
Title
Behavior of different monocomponent endoglucanases on the accessibility and reactivity of dissolving-grade pulps for viscose process
Abstract

Three different commercial monocomponent endoglucanases, with and without a cellulose-binding domain (CBD) and differences in their glycosidic hydrolysis mechanisms, were compared with respect to their ability to enhance the accessibility and reactivity of dissolving-grade pulps for viscose production. Hardwood (eucalyptus) and softwood (mixture of Norway spruce and Scots pine) commercial dried and never-dried bleached sulfite dissolving pulps were used for this purpose. The effects of the enzymatic treatments on pulps were studied by reactivity, according to Fock's method, and viscosity measurements, and recording of molecular weight distributions. Among the different assayed enzymes, endoglucanase with a CBD and an inverting hydrolysis mechanism was found to be the most effective in increasing the reactivity of both pulps. Simultaneously, the viscosity decreased, being more marked for softwood dissolving pulp. A narrower molecular weight distribution, with a great reduction in the amount of long-chain cellulose molecules was observed in both pulps, being more pronounced for softwood dissolving pulp. By contrast, endoglucanase without a CBD and a retaining hydrolysis mechanism showed a barley enhancement of the studied properties. The effects of the different endoglucanase treatments were more pronounced when never-dried dissolving pulps were used.

Keywords
Hardwood; Softwood; Dissolving-grade pulp; Endoglucanase (EG); Cellulose-binding domain (CBD); Reactivity; Viscosity; Molecular weight distribution (MWD)
First Page Preview
Behavior of different monocomponent endoglucanases on the accessibility and reactivity of dissolving-grade pulps for viscose process
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 47, Issue 7, 8 December 2010, Pages 355–362
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering