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Destabilization of the emulsion formed during the enzyme-assisted aqueous extraction of oil from soybean flour

Paper ID Volume ID Publish Year Pages File Format Full-Text
17538 42676 2009 8 PDF Available
Title
Destabilization of the emulsion formed during the enzyme-assisted aqueous extraction of oil from soybean flour
Abstract

Soybean oil extraction from soybean flour by a neutral metallo-endopeptidase enzyme-assisted aqueous extraction process, optimized for effectiveness in reducing oil content of the solid residue, yields a small fraction of the oil as free oil whereas most is emulsified in a cream layer. Analysis of the cream showed the presence of peptides/proteins and phospholipids, either of which could serve as emulsifiers. Several demulsification treatments targeting these components – protease addition, phospholipase addition, and acidification (pH 4.5) – were evaluated for effectiveness against the targeted emulsifiers and in conversion of the cream to free oil.Acidification increased the oil yield from 2% to 83%. After a two-stage enzymatic demulsification process with an alkaline endopeptidase, the oil recovery increased to 95%. A lysophospholipase A1 treatment at pH 4.5 provided complete conversion of emulsified to free oil. The phospholipids (PL) present in the original and post-treatment cream were quantified using phosphorus-31 nuclear magnetic resonance spectroscopy. PL contained phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) and their lyso-derivatives. Protein size distribution, PL proportions and fatty acid composition changed after the demulsification treatments. Recycle of the endopeptidase is feasible as more than 90% of its activity was retained.

Keywords
Emulsion; Soy oil; Protease; Phospholipase; Demulsify
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Destabilization of the emulsion formed during the enzyme-assisted aqueous extraction of oil from soybean flour
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 45, Issue 1, 8 July 2009, Pages 28–35
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us