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Continuous production of oligoglucuronans by immobilized glucuronan lyase

Paper ID Volume ID Publish Year Pages File Format Full-Text
17541 42676 2009 5 PDF Available
Title
Continuous production of oligoglucuronans by immobilized glucuronan lyase
Abstract

A glucuronan lyase was incubated with sepharose matrices pre-activated with N-hydroxysuccinimide (NHS), cyanogen bromide (CNBr) or epoxy. The CNBr- and NHS-activated gels showed satisfactory immobilization yields whereas no enzyme could be immobilized using the epoxy coupling group. Glucuronan lyase immobilized on CNBr-gel ensured rapid conversion of several glucuronans into oligomers with an enzymatic activity identical to that of the free enzyme. As classically observed when using free enzymes, the acetylation degree of glucuronan limited enzyme activity. Nevertheless, this immobilized system made it easier to obtain accurately oligomers with different polymerization degrees, notably in modifying the glucuronans residence times in column. Thus oligoglucuronan pools with polymerization degrees between 2 and 25 could be obtained with a productivity ranging from 120 mg h−1 to 1.2 g h−1 using 0.9 ml of chromatographic gel with immobilized glucuronan lyase. This methodology opens the way to continuous and large oligoglucuronan productions.

Keywords
Glucuronan lyase; Immobilized enzyme; Oligosaccharide
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Continuous production of oligoglucuronans by immobilized glucuronan lyase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 45, Issue 1, 8 July 2009, Pages 48–52
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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