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Effects of drying-induced fiber hornification on enzymatic saccharification of lignocelluloses ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
17579 42680 2011 8 PDF Available
Title
Effects of drying-induced fiber hornification on enzymatic saccharification of lignocelluloses ☆
Abstract

This study investigated the effect of fiber hornification during drying on lignocellulosic substrate enzymatic saccharification. Two chemically pretreated wood substrates and one commercial bleached kraft hardwood pulp were used. Heat drying at 105 and 150 °C and air drying at 50% RH and 23.8 °C for different durations were applied to produce substrate with various degrees of hornification. It was found that substrate enzymatic digestibilities (SEDs) of hornified substrates made from the same never-dried sample correlate very well to an easily measurable parameter, water retention value (WRV), and can be fitted by a Boltzmann function. The hornification-produced SED reduction at a given degree of hornification as the percentage of the total SED reduction when the substrate is completely hornified depends on two parameters. The first is WRV¯, which is primarily a function of the effective enzyme molecule size, and Δ, which is related to the substrate pore size distribution shape. The low values of SEDCH, SED of a completely hornified substrate, obtained from curve fittings for the three sets of samples studied, suggest that enzyme accessibility to cellulose is mainly through the pores in the cell wall rather than substrate external surface. The SEDs of hornified substrates were found to correlate to Simons’ staining measurements well. A new parameter was proposed to better correlate enzyme accessibility to cellulose using the two-color Simons’ staining technique.

Keywords
Fiber hornification; Recycled fiber/waster paper; Drying; Enzyme accessibility; Enzymatic hydrolysis/saccharification; Water retention value (WRV)
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Effects of drying-induced fiber hornification on enzymatic saccharification of lignocelluloses ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 48, Issue 1, 5 January 2011, Pages 92–99
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us