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Development of an amperometric enzyme electrode biosensor for sterigmatocystin detection

Paper ID Volume ID Publish Year Pages File Format Full-Text
17582 42681 2010 8 PDF Available
Title
Development of an amperometric enzyme electrode biosensor for sterigmatocystin detection
Abstract

A simple, rapid and highly sensitive electrochemical biosensor for the detection of sterigmatocystin (ST) based on a new enzyme named aflatoxin-oxidase (AFO), which was cloned from Armillariella tabescens and expressed in Pichia pastoris, was developed. The enzyme was immobilized onto chitosan-single-walled carbon nanotubes (CS-SWCNTs) modified Au electrode via electrostatic interactions and hydrophobic interactions. The fabricated procedures of the biosensor were characterized with Fourier transform-infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and electrochemical techniques. Effects of some experimental variables such as pH, applied potential, and temperature on the response of the biosensor were investigated. The CS-SWCNTs nanocomposite matrix could provide a favorable microenvironment for AFO to realize the direct electron transfer on the underlying electrode. The enzymatic electrode exhibited an excellent electrocatalytic response to ST, long-term stability, and good reproducibility. The linear range of ST determination was from 10 to 1480 ng mL−1 with a detection limit of 3 ng mL−1 based on the signal-to-noise ratio of 3. The kinetic parameters such as α (charge transfer coefficient), Ks (electron transfer rate constant), KMapp (the apparent Michaelis–Menten constant), and Ea (activity energy) were evaluated to be 0.4, 0.9 ± 0.02 s−1, 7.8 μM and 38.8 kJ mol−1, respectively.

Keywords
Sterigmatocystin; Aflatoxin-oxidase; Single-walled carbon nanotubes; Chitosan; Direct electron transfer; Electrochemical biosensor
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Development of an amperometric enzyme electrode biosensor for sterigmatocystin detection
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 47, Issue 4, 6 September 2010, Pages 119–126
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us