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Kinetics of thermal inactivation of free Aureobasidium pullulans fructosyltransferase

Paper ID Volume ID Publish Year Pages File Format Full-Text
17584 42681 2010 6 PDF Available
Title
Kinetics of thermal inactivation of free Aureobasidium pullulans fructosyltransferase
Abstract

A batch catalytic process of fructooligosaccharide production with the initial sucrose concentration of 700 g dm−3 was carried out in the temperature interval of 67.5–78 °C to investigate the thermal stability of free Aureobasidium pullulans fructosyltransferase. Inactivation experiments were carried out also for a saccharide-free solution in the temperature interval of 53.1–71 °C so that a stabilizing effect of saccharides contained in the reaction mixture could be assessed. A model of enzyme inactivation kinetics based on a two-step sequential mechanism was developed and verified for both media using the multitemperature evaluation of inactivation data. The model was used to predict the operational lifetime and optimal operational temperature of the biocatalyst. It was found that the use of the concentrated sucrose solution increases the operational temperature by about 15 °C compared to a dilute aqueous solution. The value of 47.5 °C was estimated as an optimal temperature at which the enzyme operational lifetime would be 1 year.

Keywords
Fructosyltransferase; Thermal stability; Biocatalyst operational lifetime; Inactivation; Kinetics; Modelling
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Kinetics of thermal inactivation of free Aureobasidium pullulans fructosyltransferase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 47, Issue 4, 6 September 2010, Pages 134–139
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us