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Biosensor based on pequi polyphenol oxidase immobilized on chitosan crosslinked with cyanuric chloride for thiodicarb determination

Paper ID Volume ID Publish Year Pages File Format Full-Text
17587 42681 2010 6 PDF Available
Title
Biosensor based on pequi polyphenol oxidase immobilized on chitosan crosslinked with cyanuric chloride for thiodicarb determination
Abstract

A novel pequi fruit (Caryocar brasiliense Camb.) homogenate source of polyphenol oxidase was obtained and immobilized in chitosan crosslinked with cyanuric chloride (CHcych-PPO). A biosensor was developed and used for the square-wave voltammetric determination of thiodicarb. Several parameters were investigated to evaluate the performance of this biosensor in the presence of hydroquinone and thiodicarb. The best response was obtained using 70:20:10% (w/w/w) of graphite powder:Nujol:CHcych-PPO, 250 U mL−1 of PPO and phosphate buffer solution (0.1 M; pH 7.0) with frequency, pulse amplitude and scan increment of 10 Hz, 150 mV, and 15 mV, respectively. Under optimized operational conditions the thiodicarb concentration was linear in the range of 3.75 × 10−7 to 2.23 × 10−6 M with a detection limit of 1.58 × 10−7 M. The biosensor was applied in the determination of thiodicarb in fresh fruit and vegetable samples and the results compared with those obtained using high-performance liquid chromatography.

Keywords
Biosensor; Thiodicarb; Polyphenol oxidase; Pequi fruit
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Biosensor based on pequi polyphenol oxidase immobilized on chitosan crosslinked with cyanuric chloride for thiodicarb determination
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 47, Issue 4, 6 September 2010, Pages 153–158
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us