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A simple fermentation strategy for high-level production of recombinant phytase by Pichia pastoris using glucose as the growth substrate

Paper ID Volume ID Publish Year Pages File Format Full-Text
17592 42682 2009 4 PDF Available
Title
A simple fermentation strategy for high-level production of recombinant phytase by Pichia pastoris using glucose as the growth substrate
Abstract

The methylotrophic yeast Pichia pastoris has become a popular host for the production of recombinant protein under the tight control of methanol-induced alcohol oxidase promoter (PAOX1), which is, however, strongly repressed by glucose at the transcription level. A real-time parameter-based controlled glucose feed strategy (rpcGFS) is developed here to realize Pichia high-cell density culture. It allows for the high-level production of recombinant phytase by P. pastoris YY113 using glucose as the growth substrate instead of glycerol. As a result, high phytase production (2200 FTU phytase ml−1) and yield (0.25 FTU ml−1[phytase] g−1[methanol]) are achieved successfully, respectively, after 100 h methanol induction by this novel simple fermentation strategy.

Keywords
Pichia pastoris; Phytase; Glucose; Feeding strategy
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A simple fermentation strategy for high-level production of recombinant phytase by Pichia pastoris using glucose as the growth substrate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 44, Issue 4, 6 April 2009, Pages 185–188
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us