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Formation of ethyl β-xylopyranoside during simultaneous saccharification and co-fermentation of paper sludge

Paper ID Volume ID Publish Year Pages File Format Full-Text
17594 42682 2009 7 PDF Available
Title
Formation of ethyl β-xylopyranoside during simultaneous saccharification and co-fermentation of paper sludge
Abstract

An unexpected product was detected during simultaneous saccharification and co-fermentation (SSCF) of paper sludge using added commercial cellulase (Spezyme CP) by Saccharomyces cerevisiae RWB222, S. cerevisiae D5A, and Zymomonas mobilis 8b. Based on glycosyl composition analysis, linkage analysis and NMR analysis, the compound was identified as ethyl β-xylopyranoside (EXP). The carbon mass balance analysis showed up to 25% of xylan originally present in paper sludge was converted to EXP. EXP formation was found in simultaneous saccharification of beech wood xylan as well, and later proved to be produced by the Trichoderma reesei derived cellulase and hemicellulase mixture (Spezyme CP) during the course of xylan hydrolysis in the presence of ethanol, and its production increased with an increased concentration of ethanol, xylan, and T. reesei enzyme. Similar condensation reactions were also observed with other alcohols. These alcoholysis reactions were found to be reversible. Thermoanaerobacterium saccharolyticum was found to be able to degrade EXP.

Keywords
Simultaneous saccharification and co-fermentation; Simultaneous saccharification and fermentation; Ethyl β-xylopyranoside; Paper sludge; Xylan; T. reesei; Ethanol; Spezyme CP
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Formation of ethyl β-xylopyranoside during simultaneous saccharification and co-fermentation of paper sludge
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 44, Issue 4, 6 April 2009, Pages 196–202
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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