fulltext.study @t Gmail

Involvement of an l-amino acid oxidase in the activity of the marine bacterium Pseudoalteromonas flavipulchra against methicillin-resistant Staphylococcus aureus

Paper ID Volume ID Publish Year Pages File Format Full-Text
17611 42683 2010 7 PDF Available
Title
Involvement of an l-amino acid oxidase in the activity of the marine bacterium Pseudoalteromonas flavipulchra against methicillin-resistant Staphylococcus aureus
Abstract

In this study, a yellow pigmented bacterial strain, designated C2, was recently isolated from the encrusting pore coral Montipora aequituberculata, which was collected from the Kenting coast of Southern Taiwan. Phylogenetic analyses based on 16S rRNA gene sequences indicate that strain C2 belongs to the genus Pseudoalteromonas and that its only closest neighbor is Pseudoalteromonas flavipulchra NCIMB 2033T strain. Based on the antibiogram assay, strain C2 shows potent antibacterial activity against several methicillin-resistant Staphylococcus aureus (MRSA) strains. Strain C2 can synthesize an antibacterial protein with a molecular mass of 60 kDa, and its isoelectric point is approximately 9.4. Liquid chromatography–tandem mass spectrometry analyses reveal close similarity of this antibacterial protein with the antibacterial protein AlpP from the marine bacterium Pseudoalteromonas tunicata D2. This study explores the nature of this antibacterial protein such as l-amino acid oxidase with broad substrate specificity. The enzyme is most active with l-Lys, l-Met, l-Glu, l-Leu, l-Gln, l-Tyr and l-Phe, and the hydrogen peroxide generated by its catalysis mediates antibacterial activity. This is the first report on a marine bacterium P. flavipulchra producing antibacterial activity by generating hydrogen peroxide through its enzymatic activity of l-amino acid oxidase against MRSA.

Keywords
l-Amino acid oxidase; Antibacterial activity; Hydrogen peroxide; Pseudoalteromonas flavipulchra; Methicillin-resistant Staphylococcus aureus
First Page Preview
Involvement of an l-amino acid oxidase in the activity of the marine bacterium Pseudoalteromonas flavipulchra against methicillin-resistant Staphylococcus aureus
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 47, Issues 1–2, 5 July 2010, Pages 52–58
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us