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Application of continuous stirred membrane reactor to 3-cyanopyridine bioconversion using the nitrile hydratase–amidase cascade system of Microbacterium imperiale CBS 498-74

Paper ID Volume ID Publish Year Pages File Format Full-Text
17614 42684 2010 7 PDF Available
Title
Application of continuous stirred membrane reactor to 3-cyanopyridine bioconversion using the nitrile hydratase–amidase cascade system of Microbacterium imperiale CBS 498-74
Abstract

The bioconversion of 3-cyanopyridine using the in situ nitrile hydratase–amidase cascade system of resting Microbacterium imperiale CBS 498-74 cells was investigated in an ultrafiltration-membrane reactor, operated in either batch or continuous mode. The effects of operating conditions such as the amount of biocatalyst, substrate concentration, substrate feeding rate, mean residence time, and enzyme-to-substrate ratio, were investigated with the aim of achieving almost 100% substrate conversion and high reactor productivity. As a result, it was found that the NHase–AMase cascade system could be adequately exploited in a continuous reactor configuration. The differing temperature dependence of nitrile hydratase and amidase kinetics enabled the operational parameters to be module d to ensure (i) nitrile hydratase operational stability (at 5 °C), and (ii) 100% conversion of 3-cyanopyridine into nicotinic acid, or, alternatively, (iii) enrichment of the effluent stream with the intermediate nicotinamide (up to 80% conversion). It was possible to select operating conditions that allowed long periods of operation (at least 100 h) at a constant flow-rate without enzyme activity loss.

Keywords
AMase, amidase; NHase, nitrile hydratase; 3-cnp, 3-cyanopyridine; CSMR, continuous stirred UF-membrane reactor; DCW, dry cell weightNitrile hydratase–amidase cascade system; 3-Cyanopyridine bioconversion; Nicotinamide; Nicotinic acid; Continuous stirred m
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Application of continuous stirred membrane reactor to 3-cyanopyridine bioconversion using the nitrile hydratase–amidase cascade system of Microbacterium imperiale CBS 498-74
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 47, Issue 3, 5 August 2010, Pages 64–70
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us