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Hydrolysis efficiency and enzyme adsorption on steam-pretreated spruce in the presence of poly(ethylene glycol)

Paper ID Volume ID Publish Year Pages File Format Full-Text
17617 42684 2010 7 PDF Available
Title
Hydrolysis efficiency and enzyme adsorption on steam-pretreated spruce in the presence of poly(ethylene glycol)
Abstract

Enzymatic hydrolysis of lignocellulosic substrates is one of the limiting steps in second generation bio-ethanol production. The effects of addition of poly(ethylene glycol) 4000 (PEG) on the efficiency and adsorption of cellulase components during the 72-h hydrolysis of steam-pretreated spruce were investigated. It was found that addition of PEG increased the amount of the enzyme components in the hydrolysis supernatant to varying degrees. As an effect of PEG addition the concentration of free proteins increased by 36% and the free overall cellulolytic activity (FPA) in the liquid fraction by 51%. After 72-h hydrolysis with PEG 48–68% higher activities towards 4-methylumbelliferyl-saccharide substrates were recovered. The results suggest that the effect of PEG is based on the decrease of non-productive enzyme binding. The structure of the hydrolysis residues was studied by 31P NMR.

Keywords
Lignocellulose; Cellulase; Hydrolysis; Enzyme adsorption; Poly(ethylene glycol)
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Hydrolysis efficiency and enzyme adsorption on steam-pretreated spruce in the presence of poly(ethylene glycol)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 47, Issue 3, 5 August 2010, Pages 84–90
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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