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Identification of endoglucanases, xylanases, pectinases and mannanases in the multi-enzyme complex of Bacillus licheniformis SVD1

Paper ID Volume ID Publish Year Pages File Format Full-Text
17621 42684 2010 7 PDF Available
Title
Identification of endoglucanases, xylanases, pectinases and mannanases in the multi-enzyme complex of Bacillus licheniformis SVD1
Abstract

Micro-organisms that degrade plant biomass require a range of enzymes to effectively degrade such substrates into sugar monomers. Some organisms secrete a host of free, extracellular enzymes while others produce a multi-enzyme complex (MEC) consisting of a variety of these enzymes. This study examined the cellulolytic and hemi-cellulolytic system of Bacillus licheniformis SVD1 with respect to the presence of key enzymes within the MEC found in this organism. When cultured on birchwood xylan, the MEC in B. licheniformis SVD1 was found to contain two endoglucanases (at 25–27 kDa, 30–32 kDa), seven xylanases (at 20 kDa, 38 kDa, 42 kDa, 45 kDa, 48 kDa, 54 kDa and 70 kDa), two mannanases (25 kDa, 40–42 kDa) and one pectinase (a pectate lyase at 70–72 kDa). Identification of enzymes was based on zymogram analysis. The MEC contained 17 different protein species based on SDS-PAGE analysis and 9 of these proteins could be identified through zymograms. Further work will be conducted to confirm the identity of other proteins in the MEC. The crude extract contained a further endoglucanase of 55 kDa, a mannanase of 53 kDa and a pectin methyl esterase of 38 kDa, indicating that not all enzymes are incorporated into the MEC. Understanding the composition of a hemi-cellulolytic system when cultured on a substrate may assist in utilising such a system for the synergistic degradation of complex lignocellulose substrates.

Keywords
Cellulosome; Hemi-cellulolytic enzymes; Lignocellulose; Zymograms
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Identification of endoglucanases, xylanases, pectinases and mannanases in the multi-enzyme complex of Bacillus licheniformis SVD1
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 47, Issue 3, 5 August 2010, Pages 112–118
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us