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A simple, sensitive and green bienzymatic UV-spectrophotometric assay of amoxicillin formulations

Paper ID Volume ID Publish Year Pages File Format Full-Text
17644 42686 2010 5 PDF Available
Title
A simple, sensitive and green bienzymatic UV-spectrophotometric assay of amoxicillin formulations
Abstract

A simple, fast, sensitive and inexpensive UV-spectrophotometric method for the determination of amoxicillin in pharmaceutical preparations has been developed based on two enzymatic reactions. In this method, d-4-hydroxyphenylglycine side chain of amoxicillin was selectively cleaved off by penicillin acylase. Subsequently, it was reacted with 2-oxoglutarate, by the catalysis of d-phenylglycine aminotransferase, to yield the product with high UV absorption namely 4-hydroxybenzoylformate. The amount of amoxicillin was then determined as a change in absorbance at 335 nm. In this work, the assay conditions were studied and optimized and the method was validated. The calibration curve presented an excellent linearity with r2 of 0.9998 (0–100 μM amoxicillin). Detection and quantitation limits were 0.77 and 2.55 μM, respectively. Good accuracy and precision were obtained when the method was tested with amoxicillin capsules and powder for oral suspension. No interference from common excipients in the formulations or degradation products was observed. Finally, since all procedures were performed without the use of any organic solvents or hazardous chemicals which were detrimental to the environment and had a low consumption of reagents, this proposed assay was an ideal green analytical method suitable for the quality control of amoxicillin in pharmaceuticals.

Keywords
Enzymatic method; Amoxicillin; Spectrophotometry
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A simple, sensitive and green bienzymatic UV-spectrophotometric assay of amoxicillin formulations
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 46, Issues 3–4, 5 March 2010, Pages 292–296
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us