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Hypersensitive immunoassay by using Escherichia coli outer membrane with autodisplayed Z-domains

Paper ID Volume ID Publish Year Pages File Format Full-Text
17647 42686 2010 6 PDF Available
Title
Hypersensitive immunoassay by using Escherichia coli outer membrane with autodisplayed Z-domains
Abstract

In this work, the Z-domain of protein A was autodisplayed on the surface of the Escherichia coli outer membrane as a fusion protein of AIDA-1, and then layered on a microplate in order to develop a hypersensitive immunoassay. By using microplates with controlled hydrophilicity the formation of outer membrane layer was carried out, and the driving force for the layer formation was proven to be a hydrophobic interactions. Through the orientation control of the immobilized antibodies by using autodisplayed Z-domains, the limit of detection (LOD) and the sensitivity of this new immunoassay were determined to have improved as much as 30-fold compared to the conventional ELISA. The applicability of the new immunoassay for medical diagnosis was demonstrated by the detection of C-reactive protein (CRP) which is known to be a biomarker protein for inflammatory diseases.

Keywords
Immunoassay; Z-domain; Orientation control; C-reactive protein; Autodisplay; Outer membrane
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Hypersensitive immunoassay by using Escherichia coli outer membrane with autodisplayed Z-domains
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 46, Issues 3–4, 5 March 2010, Pages 309–314
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us