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Expression and high-yield production of extremely thermostable bacterial xylanaseB in Aspergillus niger

Paper ID Volume ID Publish Year Pages File Format Full-Text
17659 42687 2008 4 PDF Available
Title
Expression and high-yield production of extremely thermostable bacterial xylanaseB in Aspergillus niger
Abstract

An extremely thermostable xylanaseB gene from hyperthermophilic bacterium Thermotoga maritima was ligated to pGHE vector and expressed in filamentous fungus Aspergillus niger. The vector was constructed using endogenous strong gla promoter, signal sequence, and pro-sequence (NVISKR). Positive transformants of the expression vector secreted XynB with thermostable characteristics, and optimal temperature >90 °C. Yield of XynB in shaking culture reached ∼0.5 g/l. Maximal enzymatic activity occurred at day 6, and was recorded as 625 U/ml fermentation supernatant in a transformant using Remazol Brilliant Blue R-D-Xylan as substrate. mRNA levels of gla, bip, and hac1 between recombinant and control strain were detected by quantitative reverse transcription PCR. None of these three genes was regulated in the strain of xynB integration, indicating that high expression of XynB did not induce unfolded protein response.

Keywords
xynB, xylanaseB; RBB-xylan, Remazol Brilliant Blue R-D-Xylan; UPR, unfolded protein response; hph, hygromycin B phosphotransferase; gla, glucoamylase; GRAS, generally regarded as safeAspergillus niger; Thermostable xylanaseB; Fusion expression; Secretion
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Expression and high-yield production of extremely thermostable bacterial xylanaseB in Aspergillus niger
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 43, Issue 7, 10 December 2008, Pages 513–516
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us