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Regeneration of the nicotinamide cofactor using a mediator-free electrochemical method with a tin oxide electrode

Paper ID Volume ID Publish Year Pages File Format Full-Text
17671 42688 2009 6 PDF Available
Title
Regeneration of the nicotinamide cofactor using a mediator-free electrochemical method with a tin oxide electrode
Abstract

Tin (IV) oxide was made using an anodization and annealing method and was used as a working electrode in an electrochemical cofactor regeneration reaction. This material was formed with a large surface area, and by changing the preparation conditions, it was possible to control the morphology. Tin oxide has redox properties similar to those of frequently used mediators required for electron transfer between cofactors and an electrode. Therefore, by using tin oxide as a novel electrode, mediator-free electrochemical cofactor regeneration may be possible. Oxidation and reduction of the nicotinamide cofactors, NAD(P)H and NAD(P)+, were carried out under various reaction conditions. The results showed a high efficiency for oxidizing NADH over a broad range of pH and temperatures. The oxidation tendency of NADPH was also observed, and it demonstrated a similar reaction tendency as NADH. When using a tin oxide electrode, NAD+ was readily reduced to NADH, though the efficiency of this reaction was lower than for NADH oxidation. Oxidation of 2-propanol to acetone was used as a model system using alcohol dehydrogenase and the cofactor regeneration system suggested in this study. The electroenzymatic reaction showed efficient regeneration of NADP+ without a mediator.

Keywords
Tin oxide; Mediator-free system; Electrochemical reaction; Cofactor regeneration; Nicotinamide cofactor; Oxidoreductase
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Regeneration of the nicotinamide cofactor using a mediator-free electrochemical method with a tin oxide electrode
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 44, Issue 3, 5 March 2009, Pages 129–134
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us