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Vanillin bioproduction from alkaline hydrolyzate of corn cob by Escherichia coli JM109/pBB1

Paper ID Volume ID Publish Year Pages File Format Full-Text
17675 42688 2009 5 PDF Available
Title
Vanillin bioproduction from alkaline hydrolyzate of corn cob by Escherichia coli JM109/pBB1
Abstract

Hydrolysis of corn cob performed for 6 h with 0.5 N NaOH at solid/liquid ratio of 0.084 g/g allowed obtaining a hydrolyzate containing 1171 ± 34 mg/l ferulic acid and 2156 ± 63 mg/l p-coumaric acid that was used as a medium for vanillin bioproduction by the engineered strain Escherichia coli JM109/pBB1. Aiming at maximizing vanillin bioproduction, the effects of medium heat sterilization, one-stage or two-stage pre-cultivation, adaptation of the microorganism to the hydrolyzate and inoculum biomass level were investigated. Biomass pre-cultivated once in unsterilized hydrolyzate was able to effectively convert ferulic and p-coumaric acids to a mixture of vanillin, vanillic acid and vanillyl alcohol provided with the typical vanilla flavor. At initial biomass concentration of 0.5 gDM/l, maximum values of vanillin concentration (239 ± 15 mg/l), vanillin yield on consumed ferulic acid (0.66 ± 0.03 mol/mol) and vanillin volumetric productivity (10.9 ± 0.7 mg/lh) were obtained after 22 h.

Keywords
Corn cob; Bioconversion; Alkaline hydrolysis; Ferulic acid; p-Coumaric acid; Vanillin
First Page Preview
Vanillin bioproduction from alkaline hydrolyzate of corn cob by Escherichia coli JM109/pBB1
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 44, Issue 3, 5 March 2009, Pages 154–158
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering