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Synthesis, structural analysis and application of novel acarbose-fructoside using levansucrase

Paper ID Volume ID Publish Year Pages File Format Full-Text
17683 42689 2009 6 PDF Available
Title
Synthesis, structural analysis and application of novel acarbose-fructoside using levansucrase
Abstract

Acarbose-fructoside (acarbose-Fru) was newly synthesized via the acceptor reaction of a levansucrase from Leuconostoc mesenteroides B-512 FMC with acarbose and sucrose. The resultant product was separated with 10.5% purification yield via Bio-gel P-2 column chromatography and HPLC. Its structure was determined to be 1I-β-d-fructofuranosyl α-acarbose, according to the results of 1H, 13C, HSQC, and HMBC analyses. Acarbose-Fru was inhibited competitively on α-glucosidase (A. niger and baker's yeast) but mixed noncompetitively on α-amylases (A. oryzae and porcine pancreatic). Compared to acarbose, acarbose-Fru exhibited inhibition potency of 1.12 or 1.52 on A. niger α-glucosidase or A. oryzae α-amylase, respectively. Additionally, acarbose-Fru was identified as a novel substrate for dextransucrase with Km and Vmax values of 189.0 mM and 8.51 μmol/(mg min), respectively. Therefore, acarbose-Fru as a substrate might be synthesized novel acarbose derivatives by using dextransucrase.

Keywords
Acarbose; Acceptor reaction; Levansucrase; Leuconostoc mesenteroides; Dextransucrase
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Synthesis, structural analysis and application of novel acarbose-fructoside using levansucrase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 45, Issue 4, 7 October 2009, Pages 247–252
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us