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Hydroxylation of naringin by Trichoderma harzianum to dramatically improve its antioxidative activity

Paper ID Volume ID Publish Year Pages File Format Full-Text
17688 42689 2009 6 PDF Available
Title
Hydroxylation of naringin by Trichoderma harzianum to dramatically improve its antioxidative activity
Abstract

Transforming naringin using the mycelium of Trichoderma harzianum CGMCC 1523 produces two metabolites, 3′,4′,5,7-tetrahydroxy flavanone-7-rhamnoglucoside (3′-OHN) and 3′,4′,5′,5,7-pentahydroxy flavanone-7-rhamnoglucoside (3′,5′-DOHN), both of which were characterized by ESI–MS, 1H NMR and 13C NMR analyses. The time course of the biotransformation by T. harzianum showed that 3′-OHN and 3′,5′-DOHN appeared simultaneously at 6 h, and the conversion yield (32.6%) of 3′,5′-DOHN was higher (10.6%) than that of 3′-OHN at 56 h. The optimal biotransformation temperature was 30 °C, the optimal pH was 5.0, and the optimal concentration of naringin was 400 mg/l. The bigger volume of biotransformation mixture and lower shaking speed did not favor hydroxylation reactions. The radical scavenging activity of naringin at 2000 μM was 11.1%, whereas activity of 3′-OHN at 100 μM could reach 38.4%, which is 68.6 times more than naringin. Antioxidative activity of 3′,5′-DOHN was increased 13.5% at 100 μM compared to 3′-OHN.

Keywords
Naringin; Hydroxylation; 3′,4′,5,7-Tetrahydroxy flavanone-7-rhamnoglucoside; 3′,4′,5′,5,7-Pentahydroxy flavanone-7-rhamnoglucoside; Trichoderma harzianum; DPPH radical scavenging activity
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Hydroxylation of naringin by Trichoderma harzianum to dramatically improve its antioxidative activity
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 45, Issue 4, 7 October 2009, Pages 282–287
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us