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Immobilization of alliinase with a water soluble–insoluble reversible N-succinyl-chitosan for allicin production

Paper ID Volume ID Publish Year Pages File Format Full-Text
17691 42689 2009 6 PDF Available
Title
Immobilization of alliinase with a water soluble–insoluble reversible N-succinyl-chitosan for allicin production
Abstract

N-Succinyl-chitosan (NSC), a pH-sensitive polymer of reversibly soluble–insoluble characteristics with pH change, was prepared by modification of the chitosan backbone with succinic anhydride and employed as carrier for alliinase immobilization. The obtained NSC is soluble at pH above 4.8 and insoluble at pH below 4.4. The characteristics of NSC were evaluated using Fourier transform IR spectrophotometer, the X-ray diffraction spectrometry and thermogravimetric analyzer. Under an optimized condition (glutaraldehyde 0.8% (v/v), 31.2 U alliinase), the enzyme immobilization yield was 75.6%. The maximum activity of NSCA was achieved at 40 °C, pH 7, while the free enzyme exhibited maximum activity at 30 °C, pH 6. The Michaelis–Menten constant of NSCA was lower than that of free alliinase, indicating higher affinity of immobilized enzyme toward its substrate. The NSCA retained 85% of its initial activity even after being recycled 5 times. The immobilized alliinase in reversibly soluble NSC is suitable to catalyze the conversion of alliin to allicin, as active ingredient of pharmaceutical compositions and food additive.

Keywords
N-Succinyl-chitosan; Soluble–insoluble; Immobilization; Alliinase; Alliin
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Immobilization of alliinase with a water soluble–insoluble reversible N-succinyl-chitosan for allicin production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 45, Issue 4, 7 October 2009, Pages 299–304
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us