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Production of xylitol from d-xylose and glucose with recombinant Corynebacterium glutamicum

Paper ID Volume ID Publish Year Pages File Format Full-Text
17702 42690 2010 6 PDF Available
Title
Production of xylitol from d-xylose and glucose with recombinant Corynebacterium glutamicum
Abstract

The product spectrum of a soil bacterium Corynebacterium glutamicum was extended to include a functional sugar xylitol. The recombinant C. glutamicum, engineered to express the xylose reductase gene XYL1of Pichia stipitis, produced xylose reductase with a specific activity of ca. 0.6 U/mg protein. Due to the absence of xylose isomerase and xylitol dehydrogenase genes, loose catabolite repression, high NADPH regeneration capacity, and tolerance against sugar-induced osmotic stress, the recombinant biocatalyst was able to efficiently produce xylitol from d-xylose using glucose as source of reducing equivalents. A fed-batch culture-based biotransformation allowed xylitol to accumulate to a concentration of 34.4 g/L (226 mM) in the medium with the specific productivity and product yield of xylose of 0.092 g/g dry cells/h and over 97%, respectively. The molar yield of xylitol to energy source during the biotransformation reached approximately 1.6 mol of xylose/mol of glucose.

Keywords
Biotransformation; Corynebacterium glutamicum; Xylitol; Fed-batch cultivation
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Production of xylitol from d-xylose and glucose with recombinant Corynebacterium glutamicum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 46, Issue 5, 5 April 2010, Pages 366–371
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us