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Heterologous production of NpCel6A from Neocallimastix patriciarum in Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
17704 42690 2010 6 PDF Available
Title
Heterologous production of NpCel6A from Neocallimastix patriciarum in Saccharomyces cerevisiae
Abstract

The cellobiohydrolase-encoding gene from the anaerobic chytrid Neocallimastix patriciarum (Npcel6A) was heterologously produced in Saccharomyces cerevisiae. Recombinant cellulase activity was detected on soluble cellulose substrates however little activity was found on Avicel. The unusual Asn-rich linker region along with the carbohydrate-binding module (CBM) of NpCel6A was replaced with a Ser/Thr/Pro-rich linker and CBM from the TrCel6A of Trichoderma reesei. The yeast producing the hybrid cellulase had significantly lower activity on filter paper than the original NpCel6A. When the Asn-rich linker was fused to the catalytic domain of TrCel6A, significantly higher cellulase activity was found on filter paper than the original TrCel6A.

Keywords
Saccharomyces cerevisiae; Neocallimastix patriciarum; Cellulase; Cellobiohydrolase
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Heterologous production of NpCel6A from Neocallimastix patriciarum in Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 46, Issue 5, 5 April 2010, Pages 378–383
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us