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Detection of cesA mRNA from Bacillus cereus by RNA-specific amplification

Paper ID Volume ID Publish Year Pages File Format Full-Text
17706 42690 2010 6 PDF Available
Title
Detection of cesA mRNA from Bacillus cereus by RNA-specific amplification
Abstract

Cereulide is the cyclic dodecadepsipeptide responsible for the emetic-type food-borne disease caused by Bacillus cereus. It is synthesized enzymatically by non-ribosomal peptide synthetases. In this study, we established an RNA-specific amplification assay for the detection of mRNA transcribed from the cesA gene, one of the 7 genes constituting the cereulide synthetase (ces) gene cluster of emetic-type B. cereus. The assay detected as few as 1000 copies of this RNA. Examination of the total RNAs extracted from 7 strains of cereulide-producing B. cereus, 6 strains of cereulide non-producing B. cereus, 6 strains of other Bacillus species, and 10 non-Bacillus strains showed that the assay specifically detected emetic-type B. cereus strains. RNA-specific amplification using RNA extracts provided comparable results to RT-PCR assays using RNA extracts and PCR assays using DNA extracts. The RNA-specific amplification assay is suitable for the detection of mRNA transcribed from the cesA gene.

Keywords
Bacillus cereus; Cereulide; PCR; RNA-specific amplification; RT-PCR
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Detection of cesA mRNA from Bacillus cereus by RNA-specific amplification
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 46, Issue 5, 5 April 2010, Pages 391–396
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us