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SO2-catalyzed steam pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse

Paper ID Volume ID Publish Year Pages File Format Full-Text
17710 42691 2010 10 PDF Available
Title
SO2-catalyzed steam pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse
Abstract

Sugarcane bagasse is a lignocellulosic residue obtained from sugarcane milling, and a potentially interesting raw material that can be used for fuel ethanol production. In the present study, bagasse was steam pretreated at temperatures between 180 and 205 °C, with holding times of 5–10 min using SO2 as a catalyst to determine conditions that provide a good recovery of pentoses and a suitable material for enzymatic hydrolysis. Pretreatment conducted at 190 °C for 5 min gave a pentose yield of 57%, with only minor amounts of degradation compounds formed. Commercial cellulolytic enzymes were used to hydrolyze the obtained fiber fractions after pretreatment at different water-insoluble solid contents (2%, 5% and 8% WIS). The overall highest sugar yield achieved from bagasse was 87% at 2% WIS. Fermentation tests were made on both the pentose-rich hemicellulose hydrolysate obtained from the pretreatment, and the enzymatic hydrolysates obtained from the fiber fractions using the xylose-fermenting strain of Saccharomyces cerevisiae TMB3400, as well as the natural xylose-utilizing yeast Pichia stipitis CBS 6054. The pretreatment hydrolysates produced at 2% WIS as well as the enzymatic hydrolysates showed a complete glucose fermentability indicating a low toxicity to the yeasts. The best xylose conversion (more than 60%) was achieved by the strain TMB3400 at 2% WIS.

Keywords
Sugarcane bagasse; Bioethanol; Xylose; Yeast; Saccharomyces cerevisiae
First Page Preview
SO2-catalyzed steam pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 46, Issue 2, 5 February 2010, Pages 64–73
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering