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Expression analysis of an elastin-like polypeptide (ELP) in a cell-free protein synthesis system

Paper ID Volume ID Publish Year Pages File Format Full-Text
17713 42691 2010 5 PDF Available
Title
Expression analysis of an elastin-like polypeptide (ELP) in a cell-free protein synthesis system
Abstract

An elastin-like polypeptide (ELP) fusion protein was expressed in a cell-free protein synthesis system, and the expression profile was analyzed quantitatively. By selective addition of specific amino acids constituting ELP molecules, the expression level of the ELP fusion protein was improved by 1.3–1.8 times as high as positive control. This result implies that the expression amount of long repetitive polypeptides, which was dramatically decreased in vivo system, can be compensated to a degree by adding repeatedly consumed amino acids in vitro system. Presented results demonstrate the potential of cell-free protein synthesis for high-throughput study of various repetitive polypeptides.

Keywords
Cell-free protein synthesis; Elastin-like polypeptide (ELP); Protein expression profile; Recursive directional ligation (RDL) method; Repetitive polypeptides
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Expression analysis of an elastin-like polypeptide (ELP) in a cell-free protein synthesis system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 46, Issue 2, 5 February 2010, Pages 87–91
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us