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Structural correlations of activity of Clostridium acetobutylicum ATCC 824 butyrate kinase isozymes

Paper ID Volume ID Publish Year Pages File Format Full-Text
17718 42691 2010 7 PDF Available
Title
Structural correlations of activity of Clostridium acetobutylicum ATCC 824 butyrate kinase isozymes
Abstract

The Clostridium acetobutylicum genome encodes two butyrate kinases. The gene for butyrate kinase I (BKI) is adjacent to the gene for phosphotransbutyrylase and this operon serves a major role in butyrate formation. A second butyrate kinase gene encodes an enzyme (BKII) that exhibits a much higher Km for butyrate and has a more rigid specificity than BKI. We used site specific mutagenesis and protein structural tools to explore the basis of the unique properties of BKII. Several BKII mutants were created and the enzymatic properties were compared considering structural features of other kinase family members. Alignment of the amino acid sequences for the acetate, propionate, and butyrate kinases from both Archaea and Bacteria domains revealed absolute conservation at two critical arginine and two critical histidine residues. High similarity was observed at two glutamate residues. Replacements of residues H155, H183, R215, and E334 resulted in undetectable kinase activity suggesting that these residues are essential. A polar or positively charged residue is required in position 332 and a positively charged residue is required at position 150. The amino acid sequences of both BKI and BKII were homology modeled with the three-dimensional structure of acetate kinase from Methanosarcina thermophila. This is the first structural description of an n-butyrate kinase.

Keywords
Butyrate kinase; ATP; Phosphorylation; Enzyme; Mutagenesis; Gene; Bacteria
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Structural correlations of activity of Clostridium acetobutylicum ATCC 824 butyrate kinase isozymes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 46, Issue 2, 5 February 2010, Pages 118–124
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us