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Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extracts

Paper ID Volume ID Publish Year Pages File Format Full-Text
17739 42692 2008 5 PDF Available
Title
Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extracts
Abstract

Trametes trogii (BAFC 463), Coriolus versicolor var. antarcticus (BAFC 266) and Trametes villosa (BAFC 2755) are Argentinean white-rot fungi that proved to be efficient ligninolytic enzyme producers in previous studies. The aim of the present work was investigating the ability of their crude extracellular extracts for the biobleaching of loblolly pine Kraft pulp. The production of laccase, manganese peroxidase, endoxylanase and endoglucanase was also evaluated. Out of the three fungi studied, the best biobleaching results were obtained with culture supernatants from T. trogii grown for 21 days in a synthetic medium with glucose and asparagine as carbon and nitrogen sources, respectively, with the addition of 1 mM copper sulfate. Such medium rendered high level of ligninolytic enzymes: 0.25 U ml−1 of manganese peroxidase and 44 U ml−1 of laccase. A strong brightness increase was attained after the enzymatic treatment followed by H2O2. Bleaching up to 82% ISO brightness (compared with 37% in the peroxide-bleached control) was obtained. Moreover, after the enzymatic treatment, hydrogen peroxide requirements decreased (as reflected by the higher residual peroxide), and the pulp luminance reached a value of 92.5. Addition of known laccase mediators such as HBT, ABTS, and HBA did not improve the process. The supernatant did not significantly lose its bleaching capacity even after successive recovery and reapplication to unbleached pulp. Correspondingly, laccase and manganese peroxidase activities in the recovered supernatant, exhibited near 100% of their initial values.

Keywords
Biobleaching; Enzyme stability; Kraft pulp; Laccase; Mediator; Xylanase
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Preliminary studies on the biobleaching of loblolly pine Kraft pulp with Trametes trogii crude extracts
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 43, Issue 2, 5 August 2008, Pages 164–168
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us