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Clean-up and concentration of manganese peroxidases recovered during the biodegradation of Eucalyptus grandis by Ceriporiopsis subvermispora

Paper ID Volume ID Publish Year Pages File Format Full-Text
17743 42692 2008 6 PDF Available
Title
Clean-up and concentration of manganese peroxidases recovered during the biodegradation of Eucalyptus grandis by Ceriporiopsis subvermispora
Abstract

Ceriporiopsis subvermispora was utilized for the biodegradation of Eucalyptus grandis in the presence or absence of co-substrates (glucose and corn steep liquor) during 7, 14 and 28 days. The biodegraded chips were used to prepare enzymatic extracts that, after pre-treatment with activated charcoal, were enriched in activity of manganese peroxidases (MnPs) with anion-exchange chromatography followed by elution with NaCl. Samples of these extracts were then analyzed by denaturing electrophoresis in order to compare the profiles of proteins and MnPs. Depending on the biodegradation period and on the presence or absence of co-substrates in the culture, one or two major proteins (relative molecular weights of 46.8 ± 0.6 and 51.6 ± 1.0 kDa) were identified. The presence of MnPs activity was further confirmed by native electrophoresis followed by phenol red staining.

Keywords
Ceriporiopsis subvermispora; Eucalyptus grandis; Manganese peroxidases
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Clean-up and concentration of manganese peroxidases recovered during the biodegradation of Eucalyptus grandis by Ceriporiopsis subvermispora
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 43, Issue 2, 5 August 2008, Pages 193–198
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us