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Antarctic, cold-adapted β-galactosidase of Pseudoalteromonas sp. 22b as an effective tool for alkyl galactopyranosides synthesis

Paper ID Volume ID Publish Year Pages File Format Full-Text
17750 42693 2009 6 PDF Available
Title
Antarctic, cold-adapted β-galactosidase of Pseudoalteromonas sp. 22b as an effective tool for alkyl galactopyranosides synthesis
Abstract

The antarctic Pseudoalteromonas sp. 22b β-galactosidase was found to catalyze synthesis of alkyl galactopyranosides. The number of carbon atoms in C3–C6 alcohol molecules only slightly affected the yield of products. Reactions of transgalactosylation were conducted for 80 h and the maximum accumulation of their products was observed between 40 and 50 h. The highest and almost the same yields of alkyl galactosides were achieved at pH 6–9 and 10–30% water concentration. Like known mesophilic β-galactosidases, the antarctic enzyme more efficiently synthesized alkyl galactosides when reactions were carried out in mixtures of buffers and organic solvents (below 50%, v/v).

Keywords
β-Galactosidase; Psychrophilic; Alkyl galactopyranosides; Biosurfactants; Könnigs–Knorr
First Page Preview
Antarctic, cold-adapted β-galactosidase of Pseudoalteromonas sp. 22b as an effective tool for alkyl galactopyranosides synthesis
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 44, Issue 2, 5 February 2009, Pages 59–64
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering