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Textile dye degrading laccase from Pseudomonas desmolyticum NCIM 2112

Paper ID Volume ID Publish Year Pages File Format Full-Text
17751 42693 2009 7 PDF Available
Title
Textile dye degrading laccase from Pseudomonas desmolyticum NCIM 2112
Abstract

A laccase requiring optimum temperature 60 °C, pH 4.0 for the activity and having apparent molecular weight 43,000 Da was purified from Pseudomonas desmolyticum NCIM 2112 by three steps, including heating, anion exchange, and molecular sieve chromatography. The purification fold and yield of laccase obtained through Biogel P100 were 45.75 and 19%, respectively. Staining of native gel with L-dopa showed dark brown color band indicating the presence of laccase. In relation to hydroquinone, the substrate specificity of laccase was in the following order: DAB > o-tolidine > ABTS > L-dopa. The absence of monophenolase activity in eluted fractions conformed that the purified protein is laccase. This laccase showed substrate dependent optimum pH character. Effect of inhibitor and metal ion on enzyme activity was analyzed. UV–vis analysis showed the decolorization of Direct Blue-6, Green HE4B and Red HE7B in the presence of laccase. The FTIR spectral comparison between the control dye sample and the metabolites extracted after decolorization by purified laccase have confirmed degradation of these dyes. This study contributes for the structural requirement of a dye to be degradable by P. desmolyticum laccase and is important in order to optimize potential bioremediation systems for industrial textile process water treatment.

Keywords
DAB, 3-3′-diaminobenzidine tetrahydrochloride; ABTS, 2,2′-azinobis (3-ethylbenzthiazoline-6-sulfonate; DB6, Direct Blue-6; GHE4B, Green HE4B; RHE7B, Red HE7BLaccase; Degradation; Pseudomonas desmolyticum; Textile dye; FTIR
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Textile dye degrading laccase from Pseudomonas desmolyticum NCIM 2112
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 44, Issue 2, 5 February 2009, Pages 65–71
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us