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Improvement of alkaline lipase from Proteus vulgaris T6 by directed evolution

Paper ID Volume ID Publish Year Pages File Format Full-Text
17754 42693 2009 5 PDF Available
Title
Improvement of alkaline lipase from Proteus vulgaris T6 by directed evolution
Abstract

To expand the functionality of lipase from Proteus vulgaris (PVL) we have used error-prone PCR and DNA shuffling methods to create PVL mutants with improved lipase activity. One desirable mutant with three amino acids substitutions was obtained. The mutated lipase was purified and characterized. The activity of the mutant lipase EF3.3 was 3.5 times higher than that of the wild-type (WT-PVL). The mutational effect is interpreted according to a simulated three-dimensional structure for the mutant lipase. Amino acid substitution at position 102 was determined to be critical for lipase activity, while the residue at positions 197 and 229 had only marginal effect.

Keywords
Proteus vulgaris lipase; Directed evolution; DNA shuffling; Error-prone PCR
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Improvement of alkaline lipase from Proteus vulgaris T6 by directed evolution
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 44, Issue 2, 5 February 2009, Pages 84–88
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us